Table 1.
Summary of Kinetic Parameters for cocE Enzymes a
Catalyst | Kmb (μM) | kcatc (min−1) | kcat/Kmc (M−1 s−1) |
---|---|---|---|
cocE-pIX | 586 ± 63 | 415–8.3 | 11.8 □ 103–0.2 □ 103 |
cocE-pIII | 412 ± 43 | 181–3.6 | 7.3 □ 103–0.1 □ 103 |
cocEd | 0.64 ± 0.02 | 468 ± 6 | 1.2 ± 0.04 □ 107 |
See Supporting Information for procedures of kinetic experiments.
Apparent Km.
Estimated range of kcat or kcat/Km based on the possibility of 0.1–5 copies of cocE displayed per phage particle.
Values taken from ref. 9.