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. Author manuscript; available in PMC: 2006 Jan 27.
Published in final edited form as: Nat Med. 2005 Apr 24;11(5):491–498. doi: 10.1038/nm1238

Figure 4. Self-amplification of lung ceramide synthesis.

Figure 4

Figure 4

Figure 4

Figure 4

Figure 4

Effect of short-chain exogenous ceramide on endogenous ceramide species profile in the (a) lung after C12Cer (1mg/kg, 24h, black) or vehicle (gray) and in (b) bovine pulmonary artery endothelial cells (thin layer chromatography of intra-cellular ceramides detected by DAG kinase assay) after C8Cer (10 μM; n=2). (c) Effect of C12Cer on the ASMase activity in the mouse lung (24h, mean +SD, *p =0.02). Effect of neutralizing ceramide-specific antibody (4mg/kg/d) on (d) lung ceramide species (mean +SE; *p =0.002) and on (e) lung apoptosis detected by active caspase-3 (arrows, bar 50 μm) induced by VEGFR-inh (3d).