FIG. 3.
Effect of increasing levels of rimJ on papBA transcription. The strain CWZ395 containing pCWZ101 (rimJ under the control of the Plac/ara-1 promoter) and pMV101 (lacIq) was used in this experiment. IPTG was added at concentrations ranging from 0 to 1,000 μM as indicated to induce expression of rimJ. The data points indicate β-galactosidase activities measured after growth in M9 glyc at 37°C (circles), LB at 37°C (squares), and M9 glyc at 23°C (triangles). β-Galactosidase activity was measured as described in Materials and Methods. Error is expressed as ±1 standard deviation from the mean.