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In situ hybridization of BAC clones to the short arm of Hessian fly polytene chromosome X2. (A) BAC clone Mde44o9 (STS 233) green fluorescence and BAC clone 37L3 (STS 134) red fluorescence. (B) Overlapping BAC clones Hf15k1 and Hf15k2 (STS 291); overlapping green and red fluorescence appears yellow.
