Figure 1.

Time-course of physiological changes under diamide, H₂O₂ and menadione stress. Intracellular superoxide (Parts A, D and G), peroxide (Parts B, E and H) concentrations and GSH/GSSG values (Parts C, F and I) recorded under stress were factored with their counterparts found in untreated control cultures {Et_exposed (Et_control)^-1; DCF_exposed (DCF_control^-1); GSH/GSSG_exposed (GSH/GSSG_control)^-1}. Intracellular superoxide and peroxide levels were characterized by specific Et (ethidium) and DCF (dichlorofluorescein) productions, respectively, in both stress-exposed and control cultures (Additional file 1:Supplement1 for physiological changes).