5.

Elucidation of distinct mechanistic pathways at low and high degrees of functionalization. (A) Merged fluorescence microscopy images of droplets formed in the presence of supramolecular copolymers at DF = 100%, with the different ratios of the diameters compared to DF = 25% being shown on the right. The supramolecular polymers are stained using coumarin azide (magenta), while the droplet material is stained using NBD-functionalized RG₂N (cyan). (B) Partitioning coefficients of BTA and BTAClick at DF = 70% and 100% based on HPLC analysis, showing the increase in partitioning with increasing DF. (C) Schematic depiction of the proposed cytoskeleton formation driven by the strong binding of BTAClick homopolymers to the pSS component of the complex coacervates. (D) Schematic depiction of the artificial cell property landscape facilitated by supramolecular polymers of varying condenophilicity.