Table 1.

Primer sequences for mRNA analysis by real-time PCR. Sequences designed for the detection of indicated gene products by real-time PCR are presented.

Gene	Primers (human)	Amplicon	Tm
GADPH	S: 5'-CGAGATCCCTCCAAAATCAA-3';	170	60
	AS: 5'-TTCACACCCATGACGAACAT-3’		60
COX-2	S: 5'-TGCATTCTTTGCCCAGCACT-3';	146	59
	AS: 5'-AAAGGCGCAGTTTACGCTGT-3'		59
IL-1β	S: 5'-GGACAAGCTGAGGAAGATGC-3';	120	60
	AS: 5'-TCGTTATCCCATGTGTCGAA-3'		60
IL-1RA	S: 5'-GCGAGAACAGAAAGCAGGAC-3';	154	60
	AS: 5'-CCTTCGTCAGGCATATTGGT-3'		60
IL-6	S: 5'-CACAGACAGCCACTCACCTC-3';	167	59
	AS: 5'-TTTTCTGCCAGTGCCTCTTT-3'		60
IL-8/CXCL8	S: 5'-GTGCAGTTTTGCCAAGGAGT-3';	196	60
	AS: 5'-CTCTGCACCCAGTTTTCCTT-3'		59
TNF-α	S: 5'-AGCCCATGTTGTAGCAAACC-3';	134	60
	AS: 5'-TGAGGTACAGGCCCTCTGAT-3'		60
MIP-1α/CCL3	S: 5'-CTCTGCAACCAGGTCCTCTC-3';	204	60
	AS: 5'-TTTCTGGACCCACTCCTCAC-3'		60
MIP-1β/CCL4	S: 5'-AAGCTCTGCGTGACTGTCCT-3';	211	60
	AS: 5'-GCTTGCTTCTTTTGGTTTGG-3'		60
MIP-2α/CXCL2	S: 5'-TGCAGGGAATTCACCTCAAG-3';	114	56
	AS: 5'-TGAGACAAGCTTTCTGCCCA-3'		58
MIP-3β/CCL19	S: 5'-GGTGCCTGCTGTAGTGTTCA-3';	116	60
	AS: 5'-GCTTCATCTTGGCTGAGGTC-3'		60
MIP-3α/CCL20	S: 5'-GCAAGCAACTTTGACTGCTG-3';	150	60
	AS: 5'-ATTTGCGCACACAGACAACT-3'		59
MCP-1/CCL2	S: 5'-AGCAGCAAGTGTCCCAAAGA-3'	120	59
	AS: 5'-TTGGGTTTGCTTGTCCAGGT-3'		58