FIG. 4.

(A) Physical maps and icaR primer binding sites used for RT-PCR analysis in CSF41498 and the icaR::Em^r mutants. Primers KCR2 and KCR3 were designed to measure icaR transcription in ICAR mutants. (B) Comparative measurement of icaR (primers KCR 2 and KCR3 [panel A]) and gyrB (control) transcription in S. epidermidis CSF41498 and three independent icaR insertion mutants, ICAR1, ICAR2, and ICAR3. RNA was prepared from cultures grown in BHI at 37°C to the early exponential phase of the growth curve (OD₆₀₀ = 2).