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Comparative measurement of icaA, icaR, and gyrB (control) transcription in phase variants of S. epidermidis RP62A and CSF41498 and the icaR insertion mutant ICAR1. RNA was prepared from cultures grown to the mid-exponential phase of the growth curve (OD600 = 4.5) in BHI, NaCl-glucose (BHI supplemented with 4% NaCl and 0.5% glucose), and EtOH (BHI supplemented with 4% ethanol).
