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. 2002 Aug;184(16):4400–4408. doi: 10.1128/JB.184.16.4400-4408.2002

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Copyright © 2002, American Society for Microbiology

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FIG. 8. — Comparative measurement of icaA, icaR, and gyrB (control) transcription in S. epidermidis CSF41498 and ICAR1 strains complemented with plasmids pBT2 (control) and pSER4 (icaR). RNA was prepared from cultures grown to an OD₆₀₀ of 1 at 30°C in BHI and BHI EtOH (4% ethanol), with chloramphenicol (10 μg/ml) selection. Lane 1, CSF41498(pBT2) in BHI; lane 2, CSF41498(pSER4) in BHI; lane 3, CSF41498(pBT2) in BHI-EtOH; lane 4, CSF41498(pSER4) in BHI-EtOH; lane 5, ICAR1(pBT2) in BHI; lane 6, ICAR1(pSER4) in BHI; lane 7, ICAR1(pBT2) in BHI-EtOH; lane 8, ICAR1(pSER4) in BHI-EtOH.