TABLE 2.
Features of bacteriophage φ3626 ORFs, gene products, functional assignments, and amino acid sequence homologies
| ORF | Start position | Stop position | gP
|
Functional assignment | Homology(ies)b | |
|---|---|---|---|---|---|---|
| kDa | pIa | |||||
| 1 | 54 | 557 | 19.2 | 7.2 | Terminase, small subunit | φ105 (gp21), Sfi19 (gp161), bIL309 (gp38) |
| 2 | 550 | 2295 | 67.7 | 5.9 | Terminase, large subunit | φ105 (gp22 and gp23), φSLT (gp563), D3 (gp2) |
| 3 | 2296 | 3564 | 48.7 | 8.3 | Portal protein | φ105 (gp25), φSLT (gp412), D3 (gp4) |
| 4 | 3554 | 3715 | 6.4 | 9.7 | ||
| 5 | 3720 | 4325 | 23.1 | 4.7 | Putative prohead protease | φPV83 (gp41), φC31 (gp35), φPVL (gp5a) |
| 6c | 4366 | 5631 | 47.7 | 4.9 | Major capsid protein (Cps) | φ105 (gp27), bIL285 (gp44), φadh (gp395) |
| 34.3 | 4.7 | Processed form, cleaved between R114 and D115 | ||||
| 7 | 5641 | 5919 | 10.8 | 4.9 | φ105 (gp30) | |
| 8 | 5912 | 6235 | 13.2 | 8.8 | ||
| 9 | 6236 | 6685 | 17.0 | 8.7 | φ105 (gp32) | |
| 10 | 6687 | 7037 | 14.0 | 4.4 | ||
| 11c | 7054 | 7650 | 22.5 | 4.9 | Major tail protein (Tsh) | φ105 (gp34) |
| 12 | 7665 | 7979 | 11.9 | 9.7 | ||
| 12.1d | 7665 | 8485 | 32.2 | 4.3 | ||
| 13 | 8515 | 11400 | 103.8 | 10.0 | Tail tape measure protein (Tmp) | φSLT (gp1374), TP901-1 (TMP), φPVL (gp15) |
| 14 | 11401 | 12102 | 27.4 | 4.8 | PSA (gp13), bIL285 (gp53) | |
| 15 | 12102 | 15053 | 110.0 | 6.2 | PSA (gp14), bIL285 (gp54), φg1e (gp1608) | |
| 16c | 15106 | 16584 | 55.1 | 5.5 | Minor structural protein | φ105 gp42 |
| 17 | 16597 | 16905 | 12.1 | 4.8 | ||
| 18 | 16914 | 17669 | 28.2 | 4.8 | bIL170 (gpl12, accession number AF009630) | |
| 19 | 17684 | 18061 | 14.3 | 9.6 | Holin | φ105 (gp45) |
| 20 | 18149 | 19192 | 38.8 | 5.9 | Endolysin | pIP404 (ORF10), C. perfringens (ORF10C), PSA (Ply), 12826 (Ply12) |
| 21 | 19382 | 19804 | 15.9 | 4.8 | ||
| 22 | 21119 | 20070 | 41.3 | 9.9 | Integrase | T12 (Int), PSA (gp24), T270 (Int) |
| 23 | 21587 | 21135 | 17.6 | 5.2 | PSA (gp26), φSLT (gp153), A118 (gp35) | |
| 24 | 22330 | 21608 | 27.4 | 5.1 | Repressor | φSLT (gp101a), φgle (gp132), PBSX (Xre) |
| 25 | 22504 | 22722 | 8.3 | 9.2 | Cro | A2 (Cro) |
| 26 | 22749 | 23645 | 34.5 | 8.7 | φSLT (gp81a) φadh (Tec) | |
| 27 | 23680 | 23856 | 6.8 | 9.6 | Transcriptional regulator | φETA (gp13 [64]), φPV83 (gp12) |
| 28 | 23903 | 24082 | 6.7 | 9.8 | ||
| 29 | 24093 | 24299 | 7.9 | 9.2 | ||
| 30 | 24319 | 24537 | 8.5 | 10.3 | ||
| 31 | 24527 | 24853 | 1.1 | 4.5 | ||
| 32 | 24867 | 25412 | 21.3 | 9.4 | Sigma factor | C. acetobutylicum, B. subtilis, and C. perfringens |
| 33 | 25428 | 25547 | 4.6 | 8.9 | ||
| 34 | 25563 | 26315 | 29.3 | 9.4 | φETA (gp22), φSLT (gp256), rlt (gp11 [63]) | |
| 35 | 26325 | 27608 | 48.3 | 8.3 | Helicase | P1 (Ban), SPP1 (gp40), D3 (gp74) |
| 36 | 27625 | 27825 | 7.9 | 4.4 | ||
| 37 | 27825 | 28061 | 9.3 | 4.9 | ||
| 38 | 28058 | 28270 | 7.9 | 6.4 | ||
| 39 | 28263 | 28679 | 15.4 | 4.9 | SSBe | UI36 (gp141), bIL286 (SSB), A118 (SSB) |
| 40 | 28706 | 28942 | 9.1 | 4.1 | ||
| 41 | 29020 | 29295 | 10.9 | 10.0 | ||
| 42 | 29304 | 29576 | 10.5 | 10.9 | SpoIIID | C. acetobutylicum, B. subtilis, and other bacilli |
| 43 | 29638 | 30168 | 21.1 | 8.7 | ||
| 44 | 30515 | 30865 | 13.5 | 9.5 | ||
| 45 | 30858 | 31424 | 21.4 | 4.7 | ||
| 46 | 31428 | 31973 | 21.4 | 10.2 | Recombinase | L2 (gp5 [44]), Wφ (Int [34]), 186 (Int [24]) |
| 47 | 32153 | 32500 | 13.4 | 9.5 | ||
| 48 | 32533 | 32718 | 7.4 | 4.6 | ||
| 49 | 32735 | 33058 | 12.6 | 9.9 | ||
| 50 | 33039 | 33467 | 16.6 | 9.8 | φSLT (gp104b), bIL286 (gp39), φadh (gp170) | |
a
Predicted by computer analysis.
b
For reference or accession numbers, see text.
c
Experimentally shown.
d
Translational frameshift might result in two C-terminally different products (see text).
e
SSB, single-stranded-DNA binding protein.