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. 2026 Aug 28;53(1):1478. doi: 10.1007/s11033-026-12657-2

Table 2.

- Primer sequences, RefSeq accession numbers, and predicted amplicon sizes used for quantitative real-time PCR (qPCR) analysis of hepatic gene expression in healthy Swiss mice

*Gene RefSeq accession Amplicon size (bp) Forward primer (5′–3′) Reverse primer (5′–3′)
TNF-α NM_013693.3 97 ATGGCCTCCCTCTCATCAGT TTTGCTACGACGTGGGCTAC
FASN NM_007988.3 238 CGGCTTCAGGCTCTGATACTT CTATTCTCTACCGCTGGGGC
18S rRNA NR_003278.3 155 AAACGGCTACCACATCCAAG CCTCCAATGGATCCTCGTTA

*TNF-α, tumor necrosis factor alpha; FASN, fatty acid synthase; 18S rRNA, 18S ribosomal RNA. *AIN-93 M, American Institute of Nutrition 93 maintenance diet; BSF, Black Soldier Fly (H. illucens) larvae meal; AIN-93 M+BSF, AIN-93 M diet supplemented with Black Soldier Fly (H. illucens) larvae meal