Table 2.
- Primer sequences, RefSeq accession numbers, and predicted amplicon sizes used for quantitative real-time PCR (qPCR) analysis of hepatic gene expression in healthy Swiss mice
| *Gene | RefSeq accession | Amplicon size (bp) | Forward primer (5′–3′) | Reverse primer (5′–3′) |
|---|---|---|---|---|
| TNF-α | NM_013693.3 | 97 | ATGGCCTCCCTCTCATCAGT | TTTGCTACGACGTGGGCTAC |
| FASN | NM_007988.3 | 238 | CGGCTTCAGGCTCTGATACTT | CTATTCTCTACCGCTGGGGC |
| 18S rRNA | NR_003278.3 | 155 | AAACGGCTACCACATCCAAG | CCTCCAATGGATCCTCGTTA |
*TNF-α, tumor necrosis factor alpha; FASN, fatty acid synthase; 18S rRNA, 18S ribosomal RNA. *AIN-93 M, American Institute of Nutrition 93 maintenance diet; BSF, Black Soldier Fly (H. illucens) larvae meal; AIN-93 M+BSF, AIN-93 M diet supplemented with Black Soldier Fly (H. illucens) larvae meal