FIG. 3.

Transcomplementation of the yscU null mutant strain. ECL Western blot, with polyclonal antiserum raised against the secreted Yops was used to study complementation of the yscU-null mutant YPIII(pIB75) with pLS21 or pML13 in trans expressing wild-type or a Flag-tagged variant of YscU, respectively, whole-cell fractions (A) and supernatant fractions (B), respectively. The bacteria were cultured in BHI lacking calcium at 37°C, with or without 0.4 mM IPTG, as implied in the figure. Molecular mass markers are shown to the left in kilodaltons.