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. 2002 Aug;184(16):4500–4509. doi: 10.1128/JB.184.16.4500-4509.2002

FIG. 4.

FIG. 4.

The cytoplasmic domain of YscU stimulates Yop secretion. Secretion of Yops by strain YPIII(pIB102) with pML14 or pML11 expressing the C-terminal cytoplasmic domain of YscU, amino acids 264 to 354 or amino acids 211 to 354, respectively. Separation of proteins in the supernatant fractions was performed by SDS-PAGE, followed by Coomassie brilliant blue staining. The bacteria were cultured in BHI lacking calcium at 37°C, with or without 0.4 mM IPTG, as indicated in the figure. Molecular mass markers are shown to the left in kilodaltons.