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. 2002 Aug;184(16):4510–4519. doi: 10.1128/JB.184.16.4510-4519.2002

FIG. 2.

FIG. 2.

AHL production by bisR and triR mutants. Extracts of AHLs were separated by TLC, and the AHLs produced were visualized using C. violaceum CV026 as described previously (24). Lanes 1 to 3 depict extracts of AHLs which were isolated after 24 h of growth of strains A34, A549 (bisR::Tn5), and A627 (triR::Tn5), respectively. Lanes 4 to 6 depict extracts from the same strains after 48 h growth. The migration positions of various chemically synthesized standards are indicated on the left of the TLC.