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Interaction of HGFP-SAC (lanes 2 and 5), HGFP-SAC1 (lanes 3 and 6), and HGFP-SAC2 (lanes 4 and 7) with purified native L. acidophilus CWFs. Lane 1, native CWFs only; lanes 2 to 4, CWF-bound material; lanes 5 to 7, remaining nonbound material. Lanes M contain Mr reference proteins. To minimize nonspecific binding of E. coli proteins to purified CWFs, all binding experiments were performed in 50 mM Tris-HCl (pH 7.5)-150 mM NaCl.
