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Partial purification of heterologously expressed A. fulgidus β-RFAP synthase
| Purification step | Total activitya (nmol of β-RFAP/min) | Specific activity (nmol of β-RFAP/min/mg of protein) | Yield (%) | Increase in purification (n-fold) |
|---|---|---|---|---|
| Cell extract | 580 | 9.8 | 100 | 1 |
| Heat treatment | 560 | 100 | 97 | 10 |
| Hydroxyapatite | 410 | 440 | 71 | 45 |
| Phenyl Sepharose | 24 | 290 | 4 | 30 |
β-RFAP synthase assays were performed at pH 5.3 for 1 h at 70°C as described in Materials and Methods.
