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. 2002 Sep;184(17):4920–4924. doi: 10.1128/JB.184.17.4920-4924.2002

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Copyright © 2002, American Society for Microbiology

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FIG. 3. — Mutations in kinase-like motifs in RamC block function in vivo. (A) Growth of strains on solid R2YE medium. The plates show the S. coelicolor ramC null mutant N373 containing the ramC complementation plasmid pTO8 (top left), control plasmid pSETΩ (top middle), and pTO8 containing mutant alleles expressing RamC with mutations in predicted catalytic residues, including K259A (top right), D369A (bottom left), D387A (bottom middle), and D412A (bottom right). (B) Western analysis of membrane fractions of N373 containing pTO8 and pTO8 expressing the K259A, D369A, and D387A mutations in RamC.

FIG. 3. — Mutations in kinase-like motifs in RamC block function in vivo. (A) Growth of strains on solid R2YE medium. The plates show the S. coelicolor ramC null mutant N373 containing the ramC complementation plasmid pTO8 (top left), control plasmid pSETΩ (top middle), and pTO8 containing mutant alleles expressing RamC with mutations in predicted catalytic residues, including K259A (top right), D369A (bottom left), D387A (bottom middle), and D412A (bottom right). (B) Western analysis of membrane fractions of N373 containing pTO8 and pTO8 expressing the K259A, D369A, and D387A mutations in RamC.