FIG. 4.
Degradation products produced from PhoA-TM8-Cn proteins. Strain AR5087 (ftsH+) was transformed with either pCH312 (carrying PhoA; lane 1), pCH306 (carrying PhoA-TM8-C−5; lane 2), pCH309 (carrying PhoA-TM8-C30; lane 3), pCH308 (carrying PhoA-TM8-C10; lane 4), or pCH307 (carrying PhoA-TM8-C5; lane 5). Cells were induced for the lac transcription and pulse-labeled with [35S]methionine for 1 min, which was followed by a chase with unlabeled methionine for 8 min. Labeled proteins were immunoprecipitated with anti-PhoA, separated by SDS-PAGE, and visualized. PhoA∗ indicates a degradation product consisting of the PhoA domain and some TM8 residues, whereas PhoA∗∗ indicates a degradation product containing fewer TM8 residues.