Table 2:
Immunophenotype differences between various OA groups and controls in CyTOF discovery and DNA methylation-imputed validation cohorts.
| Cellular subset | OA/RP/PP frequency: CyTOF discovery cohort | HC/NP frequency: CyTOF discovery cohort | OA/RP/PP frequency: imputed validation cohort | HC/NP frequency: imputed validation cohort | OA/RP/PP: HC/NP ratio, discovery cohort | OA/RP/PP: HC/NP ratio, validation cohort | Validated difference |
|---|---|---|---|---|---|---|---|
| OA vs. HC | |||||||
| CD8+ central memory cells | 0.007±0.007 | 0.002±0.001 | 0.01±0.004 | 0.004±0.004 | 3.5 | 2.5 | Yes |
| CD4+ regulatory T cells | 0.01±0.01 | 0.005±0.002 | 0.02±0.005 | 0.006±0.004 | 2.0 | 3.3 | Yes |
| Nonclassical monocytes | 0.008±0.005 | 0.004±0.003 | 0.01±0.005 | 0.004±0.0004 | 2.0 | 2.5 | Yes |
| Basophils | 0.01±0.007 | 0.005±0.003 | 0.02±0.004 | 0.006±0.004 | 2.0 | 3.3 | Yes |
| Plasmablasts | 0.0009±0.0008 | 0.0004±0.0005 | 0.007±0.005 | 0.002±0.004 | 2.3 | 3.5 | Yes |
| Eosinophils | 0.001±0.002 | 0.0005±0.0005 | 0.007±0.005 | 0.002±0.004 | 2.0 | 3.5 | Yes |
| MAIT/NKTs | 0.02±0.01 | 0.01±0.01 | 0.007±0.006 | 0.008±0.004 | 2.0 | 0.9 | No |
| RP vs. NP | |||||||
| CD4+ central memory cells | 0.05±0.01 | 0.08±0.03 | 0.03±0.008 | 0.04±0.007 | 0.6 | 0.8 | Yes |
| CD8+ central memory cells | 0.004±0.003 | 0.01±0.007 | 0.01±0.004 | 0.011±0.004 | 0.4 | 0.9 | Yes |
| CD8+ terminal memory cells | 0.008±0.008 | 0.02±0.02 | 0.005±0.007 | 0.005±0.006 | 0.4 | 1.0 (n/s) | No |
| PP vs. NP | |||||||
| CD4+ terminal effector cells | 0.02±0.02 | 0.01±0.01 | 0.01±0.005 | 0.01±0.004 | 2.0 | 1.0 (n/s) | No |
| CD8+ terminal memory cells | 0.02±0.03 | 0.01±0.01 | 0.006±0.008 | 0.005±0.006 | 2.0 | 1.2 (n/s) | No |
HC = healthy control, RP = radiographic progressor, PP = pain progressor, NP = nonprogressor, n/s = not significant. Frequency represents mean±SD fraction total cells.