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Southern hybridization with probe for f237 phage. Lane 1, lambda ladder as size standards; lanes 2 and 3, NotI digests of KX-V237 genomic DNA. Lanes 1 and 2, ethidium bromide-stained gel; lane 3, Southern hybridization with a probe for f237 phage. The conditions for PFGE were on 1% agarose gel in 0.5× Tris-borate-EDTA buffer at 6 V/cm with pulse times of 1 to 60 s for 21 h at 14°C. The upper signal in lane 3 is the position of the well.
