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(A) DtxR binding of IRP6 requires a divalent transitional metal ion, such as Co2+. (B) Effect of DtxR concentration on formation of DtxR-DNA complexes in gel mobility shift assays. Detectable shifts of IRP6 occurred at 100 nM DtxR, while detectable shifts of IRP1 started at 5 nM DtxR. Co2+ was present in all samples at 300 μM.
