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. 2002 Sep;184(17):4846–4856. doi: 10.1128/JB.184.17.4846-4856.2002

FIG. 4.

FIG. 4.

Organization of irp6 operon. (A) Restriction map of the 6.8-kb chromosomal HindIII fragment containing the irp6 operon. Long arrows, orientations of the three ORFs; short arrows, locations and orientations of the primers used to amplify the entire irp6 region, or regions containing only the first ORF (irp6A) or the first two ORFs (irp6AB) of the irp6 operon; black bar, location of IRP6 promoter fragment isolated by the SELEX-like procedure. (B) Restriction map of library clone pSK6a, containing the 1.9-kb EcoRI fragment that hybridized to an IRP6 probe, and library clone pSK6e, containing the 2.2-kb EcoRI fragment that is adjacent to pSK6a on the chromosome. Short arrows, locations of the primers (6C2 and 6D2) used in inverse PCR to amplify the flanking sequence on the 6.8-kb HindIII fragment. (C) Restriction map of the 3.6-kb SalI-BamHI insert containing the irp6 region in plasmid pCM6ABC. The asterisk indicates that the BamHI site was generated by primer 3Q2. Restriction enzyme abbreviations: B, BamHI; E, EcoRI; H, HindIII; S, SalI; P, PstI.