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β-Galactosidase activities of C. diphtheriae strains C7(β), HC1, HC3, HC4, and HC5 carrying promoter fusion constructs pCM6 (A) and pCM1(B) cultured in HITW, HITW-Fe, and HITW with 100 μg of EDDA/ml. Overnight cultures were used for β-galactosidase activity assays, and the activity values were the averages of samples from cultures grown in triplicate. The standard deviations are shown by the error bars.
