TABLE 2.
Linkers and primers used for cycle selection
| Method | Sequence of linker or primera |
|---|---|
| HhaI selection | |
| Linker | BamHISpeI |
| 5′CGGATCCACTCTTGACCTCGACTAGTGCA3′ | |
| 3′GCGCCTAGGTGAGAACTGGAGCTGATCACGT5′ | |
| (HhaI) | |
| PCR primer PAS-A | 3′TAGGTGAGAACTGGAGCT5′ |
| HpaII selection | |
| Linker | (HpaII) XbaISpeI |
| 5′CGGATCTAGACTTGACCTCGACTAGTGCA3′ | |
| 3′CTAGATCTGAACTGGAGCTGATCACGT5′ | |
| PCR primer PAS-B | 3′TAGATCTGAACTGGAGCT5′ |
a
Boldface letters indicate the identical sequence between the linker and its specific PCR primer. Underlined letters represent target sites for indicated restriction enzymes, and protruding ends of linkers are compatible with DNA fragments generated by restriction enzymes shown in parentheses.