FIG. 6.

Effect of mutation of SPI2 genes on the surface association of SPI2 TTSS translocon proteins SseB and SseC. Wild-type Salmonella serovar Typhimurium strain NCTC 12023, ssaC mutant strain P7G2, spiC mutant strain EG10128, and spiC mutant strain EG10128 harboring pEG9127 were grown in minimal media at pH 5.8 to induce the expression of the SPI2 TTSS and the secretion of substrate proteins. Total-cell fractions and detached-protein fractions were prepared and analyzed as described in Materials and Methods.