TABLE 1.
Strains and plasmids used in this study
| Designation | Description | Reference or source |
|---|---|---|
| Plasmids | ||
| pEM25 | sspH1-cyaA in pMJH20, Apr | 24 |
| pEM30 | sspH2-cyaA in pMJH20, Apr | 24 |
| pEM80 | sseI-cyaA in pMJH20, Apr | 23 |
| pEM82 | sseJ-cyaA in pMJH20, Apr | 23 |
| pKAS32 | rpsL suicide vector for allelic exchange | 35 |
| pJAF21 | ΔsifA in pKAS32 | This study |
| pWSK29 | Low-copy-number plasmid | 40 |
| pCAS61 | pWSK29 with HA epitope tag | This study |
| pJAF111 | sseJ-HA in pCAS61, Apr | This study |
| pWSK30 | Low-copy-number plasmid | 40 |
| p2062 | M45 epitope tag in pSK+ | 12 |
| p2129 | sseJ-M45 in pWSK29, Apr | 12 |
| p2127 | spiC-M45 in pWSK30, Apr | This study |
| pEG9127 | spiC in pBAC108L, Cmr | 38 |
| pKD46 | Plasmid expressing λ Red recombinase | 8 |
| pKD4 | Plasmid with FRT-flankeda kanamycin resistance gene | 8 |
| pCP20 | Plasmid expressing the FLP recombinase | 8 |
| Salmonella serovar Typhimurium strains | ||
| NCTC 12023 | Wild type, identical to ATCC 14028s | ATCC |
| P3F4 | NCTC 12023, ssrA::mTn5 Kmr | 34 |
| P7G2 | NCTC 12023, ssaC::mTn5 Kmr | 34 |
| HH109 | NCTC 12023, ssaV::aphT Kmr | 9 |
| EG10128 | ATCC 14028s, spiC::aphT Kmr | 38 |
| CS050 | ATCC 14028s, phoN::Tn10d-Kan | S. I. Miller laboratory |
| CS401 | ATCC 14028s, phoN2 zxx::6251 Tn10d-Cm, Strepr | 24 |
| CS600 | ATCC 14028s, phoN::Tn10d-Kan, Strepr | This study |
| EM232 | CS401, ssaT::mTn5 Kmr | 24 |
| JAF204 | CS401, ΔspiC | This study |
| JAF57 | CS401, ΔsifA | This study |
a
FRT, Flp recognition target.