FIG. 1.
Primer extension analysis of the ald transcript from M. smegmatis. Total RNA (50 μg) from mc2155 cells, grown in either M-ADC-TW or M-ADC-TW supplemented with l-alanine, was annealed with primer SMALDPE and extended as described in Materials and Methods. Lanes A, C, G, and T show a dideoxy sequencing ladder of the ald gene generated with the same primer. The transcriptional start site is indicated by an arrow. The putative −10 box deduced from the consensus of mycobacterial promoter sequences is highlighted (19). The start codon (ATG) is enclosed in a box, and the putative ribosome binding site (GAAGGAG) is indicated by asterisks.