TABLE 2.
Bacterial strains used in this studya
| Strain | Genotype | Source or reference |
|---|---|---|
| 168 | trpC2 | Laboratory stock |
| BSIP1142 | trpC2 amyE::pmetI′-lacZ cat | 1 |
| BSIP1159 | trpC2 amyE::pmetK′-lacZ cat | 21 |
| BSIP1214 | trpC2 ytlI::aphA3 | 3 |
| BSIP1215 | trpC2 amyE::pytlI′-lacZ cat | pDIA5575→168 |
| BSIP1306 | trpC2 amyE::pmetE′-lacZ cat | pDIA5626→168 |
| BSIP1307 | trpC2 amyE::p(G−273→A) metE′-lacZ cat | pDIA5627→168 |
| BSIP1324 | trpC2 amyE::pkatA′-lacZ cat | pDIA5624→168 |
| BSIP1379 | trpC2 metK1 sacB::φPveg-metK+amyE::pmetI′-lacZ cat | pDIA5510→SA29 |
| BSIP1382 | trpC2 metK1 sacB::φPveg-metK+amyE::pmetE′-lacZ cat | pDIA5626→SA29 |
| BSIP1385 | trpC2 metK1 sacB::φPveg-metK+ amyE::p(G−273→A) metE′-lacZ cat | pDIA5627→SA29 |
| BSIP1386 | trpC2 yxjH′::lacZ cat | pDIA5628→168 |
| BSIP1387 | trpC2 metK1 sacB::φPveg-metK+yxjH′::lacZ cat | pDIA5628→SA29 |
| BFS1605b | trpC2 yhcL′::lacZ erm | S. Bron |
| BFS1850b | trpC2 ykrT′::lacZ erm | K. M. Devine |
| BFS1853b | trpC2 ykrZ′::lacZ erm | K. M. Devine |
| BFS2048b | trpC2 yoaD′::lacZ erm | W. Schumann |
| BFS2062b | trpC2 yrhB′::lacZ erm | W. Schumann |
| BFS2063b | trpC2 yrhA′::lacZ erm | W. Schumann |
| BFS3022b | trpC2 yitJ′::lacZ erm | S. J. Seror |
| BFS4035c | trpC2 yxjG′::lacZ erm | Y. Fujita |
| BFS4069c | trpC2 yxeQ′::lacZ erm | Y. Fujita |
| BFS4071c | trpC2 yxeO′::lacZ erm | Y. Fujita |
| BFS4073c | trpC2 yxeM′::lacZ erm | Y. Fujita |
| BFS4075c | trpC2 yxeK′::lacZ erm | Y. Fujita |
| BFS4363c | trpC2 yciB′::lacZ erm | K. Yamane |
| BFS4364c | trpC2 yciC′::lacZ erm | K. Yamane |
| BFS4451c | trpC2 ydbM′::lacZ erm | F. Kawamura |
| SA29 | 62378 with sacB::φPveg-metK+ (formerly metE+) | 30 |
| 1A607d | trpC2 metC85::Tn917 | BGSCe |
a
Arrows indicate construction by transformation. cat is the pC194 chloramphenicol acetyl-transferase gene, aphA3 is the Enterococcus faecalis kanamycin resistance gene, and erm is an erythromycin resistance gene.
b
Strain constructed as part of the EC project for the functional characterization of the B. subtilis genome (http://locus.jouy.inra.fr/cgi-bin/genmic/madbase/progs/madbase.operl).
c
Strain constructed as part of the Japanese project for functional characterization of the B. subtilis genome (http://bacillus.genome.ad.jp).
d
In the metC85::Tn917 mutant, the transposon is inserted in the metE (formerly metC) gene.
e
BGSC, Bacillus Genetic Stock Center.