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Mass spectrometric assay of CA activity in whole cells of R. eutropha wild-type strain H16 and can mutant HB1. Cells were grown in pyruvate-mineral medium under different aerations. (A) H16 grown under air. (B) H16 grown under air plus 10% (vol/vol) CO2. (C) HB1 grown under air plus 10% (vol/vol) CO2. Arrows mark the time points at which cells were added to the assay mixture.
