TABLE 3.
Role of the antisense element in stationary-phase induction and ppGpp effects on rpoS-laca
|
lac fusion
|
β-Galactosidase activity (U)
|
Activity ratio, mutant/wild type
|
||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Wild type
|
Mutant (ppGpp°)
|
|||||||||
| Promoter | Reporter | E | S + 3 | S + 3/E | E | S + 3 | S + 3/E | E | S + 3 | S + 3/E |
| lacUV5 | rpoS-lac | 5.7 | 82 | 14 | 2.1 | 6.7 | 3.2 | 0.37 | 0.08 | 0.22 |
| rpoS-lac, Δ1 | 4.7 | 65 | 14 | 1.8 | 7.5 | 4.2 | 0.38 | 0.12 | 0.30 | |
| rpoS-lac, Δ2 | 3.1 | 34 | 11 | 1.6 | 5.7 | 3.6 | 0.52 | 0.17 | 0.32 | |
| rpoS-lac, Δ3 | 13 | 73 | 5.6 | 8.4 | 62 | 7.4 | 0.65 | 0.85 | 1.3 | |
| rpoS-lac, Δ4 | 8.3 | 40 | 4.8 | 5.2 | 30 | 5.8 | 0.63 | 0.75 | 1.2 | |
| rpoS | rpoS-lac | 3.1 | 120 | 39 | 0.66 | 8.8 | 13 | 0.21 | 0.07 | 0.34 |
| rpoS-lac C469G | 7.7 | 210 | 27 | 1.4 | 47 | 34 | 0.18 | 0.22 | 1.23 | |
| rpoS-lac G549C | 14 | 360 | 26 | 3.3 | 29 | 8.8 | 0.24 | 0.08 | 0.34 | |
| rpoS-lac C469G G549C | 6.5 | 170 | 26 | 1.4 | 7.9 | 5.6 | 0.22 | 0.05 | 0.22 | |
a
All fusions listed in this table are protein fusions. See Table 2, footnote a, for definitions.