TABLE 1.
Homology between ORFs in R391 and corresponding representative prokaryotic protein sequences
ORFa | ORF product size (amino acids) | ORF location (start, stop)b | ORF product exhibits homology to: | Source | Identityc
|
Accession no. | ||
---|---|---|---|---|---|---|---|---|
No. | Name | % | Amino acids | |||||
1 | 107 | 442, 119 | ABC transporter permease protein | Lactococcus lactis | 27 | 25/92 | NP 267026 | |
2 | 106 | 790, 1110 | Y4dL; hypothetical 21.8-kDa protein | Rhizobium sp. strain NGR234 | 50 | 44/87 | NP 443821 | |
3 | 404 | 1103, 2317 | Y4dM; putative protein | Rhizobium sp. strain NGR234 | 55 | 224/405 | NP 443822 | |
4 | 66 | 2528, 2728 | Possible DNA-binding protein | Mycobacterium leprae | 34 | 17/50 | np 302574 | |
5 | int | 413 | 3986, 2745 | Integrase | SXT element; Vibrio cholerae | 99 | 410/413 | AAC72397 |
6 | 89 | 4257, 3988 | Soluble lytic murein transglycosylase | Brucella melitensis | 28 | 20/71 | np 540005 | |
7 | 324 | 5234, 4260 | StbA; putative plasmid partitioning protein | Plasmid R27; Salmonella enterica serovar Typhi | 24 | 62/252 | Q9RGU9 | |
10 | rumB | 435 | 7460, 6153 | SamB; UV protection protein | S. enterica serovar Typhi CT18 | 61 | 256/419 | np 458681 |
11 | rumA | 149 | 7878, 7429 | RumA | SXT element; Vibrio cholerae | 98 | 147/149 | AAK64594 |
13 | 302 | 8518, 9426 | DNA polymerase III (epsilon subunit) | Agrobacterium tumefaciens strain C58 | 39 | 117/300 | AAL43906 | |
14 | 104 | 9500, 9814 | Polynucleotide phosphorylase | Deinococcus radiodurans | 33 | 18/53 | np 295786 | |
15 | 308 | 9883, 10809 | NMA1157; hypothetical protein | Neisseria meningitidis | 35 | 93/261 | np 283925 | |
16 | tnpIS15 | 238 | 11635, 10919 | P22 protein | IS15; Escherichia coli | 100 | 234/234 | Q56369 |
17 | 279 | 11096, 11935 | P11 protein | IS15; Escherichia coli | 100 | 197/197 | Q56370 | |
18 | 202 | 12560, 11952 | P11 protein | IS15; Escherichia coli | 100 | 197/197 | Q56370 | |
19 | tnpIS15 | 238 | 12021, 12737 | P22 protein | IS15; Escherichia coli | 100 | 234/234 | Q56369 |
20 | aph | 271 | 12877, 13692 | Aminoglycoside phosphotransferase | Klebsiella pneumoniae | 100 | 271/271 | S16630 |
21 | tnpIS15 | 238 | 14588, 13872 | P22 protein | IS15; Escherichia coli | 100 | 234/234 | Q56369 |
22 | 199 | 14049, 14648 | P11 protein | IS15; Escherichia coli | 100 | 197/197 | Q56370 | |
23 | 210 | 14567, 15199 | Putative inner membrane protein | S. enterica serovar Typhimurium LT2 | 41 | 76/182 | NP 463357 | |
24 | 194 | 15196, 15780 | Putative cytoplasmic protein | S. enterica serovar Typhimurium LT2 | 61 | 116/188 | NP 463356 | |
25 | 947 | 15799, 18642 | Putative ATPase involved in DNA repair | S. enterica serovar Typhimurium LT2 | 62 | 585/943 | NP 463355 | |
26 | 317 | 18524, 19477 | Putative ATPase involved in DNA repair | S. enterica serovar Typhimurium LT2 | 43 | 123/280 | NP 463355 | |
27 | 171 | 19634, 20149 | Putative type II restriction enzyme, methylase subunit | S. enterica serovar Typhimurium LT2 | 56 | 96/171 | NP 463354 | |
28 | tnp391A | 225 | 20708, 21385 | OrfB of IS911 | IS911; Escherichia coli | 88 | 198/224 | AAC70114 |
29 | 1,083 | 21303, 24554 | Putative type II restriction enzyme, methylase subunit | S. enterica serovar Typhimurium LT2 | 59 | 632/1060 | NP 463354 | |
30 | 894 | 24554, 27238 | Putative cytoplasmic protein | S. enterica serovar Typhimurium LT2 | 50 | 455/896 | NP 463351 | |
31 | 746 | 27251, 29491 | Putative ATP-dependent Lon protease | S. enterica serovar Typhimurium LT2 | 85 | 576/672 | NP 463350 | |
32 | 883 | 29535, 32186 | Orf74 | Plasmid pB171; Escherichia coli | 25 | 235/920 | NP 053136 | |
33 | traI | 716 | 32341, 34491 | Hypothetical protein | Xylella fastidiosa 9a5c | 26 | 94/353 | NP 299042 |
34 | 606 | 34540, 36360 | TraG | Plasmid R27; S. enterica serovar Typhi | 30 | 186/615 | NP 458648 | |
35 | 186 | 36370, 36930 | Large repetitive protein | S. enterica serovar Typhi CT18 | 25 | 20/78 | NP 458558 | |
36 | 214 | 36908, 37552 | Hypothetical protein | Plasmid R27; S. enterica serovar Typhi | 29 | 29/98 | NP 058330 | |
37 | 195 | 38166, 37579 | Hypothetical protein | Listeria monocytogenes | 24 | 30/123 | NP 464171 | |
39 | traL | 93 | 38455, 38736 | Sex pilus assembly and synthesis protein | Plasmid pNL1; Novosphingobium aromaticivorans | 25 | 18/72 | NP 049168 |
40 | traE | 130 | 38967, 39359 | HtdE | Plasmid R478; Serratia marcescens | 25 | 29/115 | AAL27019 |
41 | traK | 300 | 39340, 40242 | HtdP | Plasmid R478; Serratia marcescens | 26 | 66/251 | AAL27020 |
42 | traB | 429 | 40242, 41531 | TrhB; putative transfer protein | Plasmid R27; S. enterica serovar Typhi | 40 | 84/209 | NP 058242 |
43 | htdD | 216 | 41528, 42178 | HtdD | Plasmid R478; Serratia marcescens | 28 | 45/158 | AAD01915 |
44 | traA | 128 | 42175, 42561 | TraA | Plasmid R27; S. enterica serovar Typhi | 24 | 21/85 | NP 058247 |
45 | 106 | 42922, 42602 | Putative acetyltransferase | S. enterica serovar Typhimurium LT2 | 45 | 41/90 | NP 463183 | |
46 | 88 | 43365, 43099 | Hypothetical protein | Escherichia coli O157:H7 | 44 | 40/89 | NP 289961 | |
47 | 125 | 43920, 43543 | SMA0431, hypothetical protein | Rhizobium meliloti | 27 | 31/114 | Q930G9 | |
48 | tnp391B | 305 | 45323, 44406 | Transposase | Listonella anguillarum | 66 | 201/301 | AAA81774 |
49 | 107 | 46010, 45687 | Glycosyl transferase | Streptomyces fradiae | 22 | 21/95 | AAF00214 | |
50 | 82 | 46700, 46452 | Hypothetical protein G | Tn1404 Pseudomonas sp. strain R9 | 60 | 15/25 | AAD47995 | |
51 | 508 | 45742, 47268 | Sulfate permease | Tn1404 Pseudomonas sp. strain R9 | 80 | 401/497 | AAD47994 | |
52 | 283 | 47280, 48131 | Hypothetical protein B | Tn1404 Pseudomonas sp. strain R9 | 38 | 109/283 | AAD47991 | |
53 | 149 | 48604, 48155 | Conserved hypothetical protein | Pseudomonas aeruginosa | 36 | 53/146 | NP 250418 | |
54 | 169 | 49144, 48635 | Conserved hypothetical protein | Pseudomonas aeruginosa | 35 | 39/109 | NP 249266 | |
56 | htdT | 230 | 49588, 50280 | HtdT; putative transfer protein | Plasmid R27; S. enterica serovar Typhi | 26 | 53/197 | NP 058240 |
57 | traC | 799 | 50280, 52679 | TraC | Plasmid R27; S. enterica serovar Typhi | 27 | 239/869 | NP 058238 |
59 | trhF | 163 | 53024, 53515 | TrhF | Plasmid R27; S. enterica serovar Typhi | 28 | 36/127 | NP 058222 |
60 | traW | 375 | 53523, 54650 | Putative transfer protein, pilus formation | Plasmid R27; S. enterica serovar Typhi | 27 | 84/303 | NP 058221 |
61 | traU | 342 | 54634, 55662 | TraU | Plasmid pNL1; Novosphingobium aromaticivorans | 36 | 125/339 | NP 049157 |
62 | traN | 1,230 | 55665, 59357 | TraN | Genomic island STI1; S. enterica serovar Typhimurium | 33 | 418/1253 | AAK02035 |
64 | 220 | 59951, 60613 | DNA ligase | Escherichia coli O157:H7 | 25 | 31/124 | NP 288973 | |
65 | 200 | 61306, 60704 | Lipoprotein | Haemophilus ducreyi | 21 | 30/139 | AAB48950 | |
66 | 112 | 61662, 62000 | AnsG oxidoreductase | Streptomyces collinus | 27 | 19/68 | AAD31836 | |
67 | 139 | 62016, 62435 | Single-strand binding protein | Plasmid pLP231a; Escherichia coli | 38 | 52/135 | C38487 | |
68 | 272 | 62515, 63333 | Recombination protein Bet | Bacteriophage 933W; Escherichia coli O157:H7 | 55 | 108/195 | NP 049474 | |
69 | 338 | 63621, 64637 | Hypothetical protein | Xylella fastidiosa 9a5c | 32 | 51/155 | NP 298936 | |
70 | 358 | 64730, 65806 | Probable cobalamin biosynthesis protein | Sinorhizobium meliloti | 30 | 87/281 | NP 386778 | |
71 | 255 | 65806, 66573 | Molybdate metabolism regulator-related protein | Deinococcus radiodurans | 31 | 33/105 | NP 386778 | |
72 | 317 | 66672, 67625 | Hypothetical protein | Plasmid R27; S. enterica serovar Typhi | 20 | 41/205 | NP 058418 | |
74 | 551 | 68197, 69852 | Hypothetical protein | Plasmid R27; S. enterica serovar Typhi | 21 | 138/636 | NP 058417 | |
75 | 165 | 69938, 70435 | RadC; putative DNA repair protein | Vibrio cholerae | 56 | 86/151 | NP 231421 | |
78 | 357 | 70867, 71940 | Hypothetical protein | Plasmid R27; S. enterica serovar Typhi | 30 | 113/375 | NP 058415 | |
79 | 235 | 72030, 72737 | Hypothetical protein | SXT element; Vibrio cholerae | 80 | 61/76 | AAK95994 | |
80 | 180 | 73088, 73630 | Probable oxidoreductase | Agrobacterium tumefaciens | 27 | 35/126 | NP 396434 | |
81 | traF | 314 | 74368, 75312 | TraF | SXT element; Vibrio cholerae | 97 | 306/314 | AAK 95986 |
82 | traH | 462 | 75315, 76703 | TraH | Genomic island STI1; Salmonella enterica serovar Typhimurium | 42 | 189/449 | AAK02038 |
83 | traG | 1,189 | 76707, 80276 | Putative pilus assembly protein | Genomic island STI1; Salmonella enterica serovar Typhimurium | 30 | 375/1214 | AK02037 |
84 | merR | 129 | 80374, 80763 | MerR | IncJ element pMERPH; Shewanella putrefaciens | 100 | 557/557 | |
85 | merT | 107 | 80848, 81171 | MerT | IncJ element pMERPH; Shewanella putrefaciens | 97 | 99/102 | Q54462 |
86 | merP | 91 | 81256, 81531 | MerP | IncJ element pMERPH; Shewanella putrefaciens | 98 | 90/91 | Q54463 |
87 | merC | 137 | 81541, 81954 | MerC | IncJ element pMERPH; Shewanella putrefaciens | 98 | 135/137 | CAA89056 |
88 | merA | 557 | 81992, 83665 | MerA; mercuric reductase | IncJ element pMERPH; Shewanella putrefaciens | 100 | 557/557 | Q54465 |
89 | 143 | 84202, 83771 | Hypothetical protein | Listeria innocua | 26 | 23/86 | CAC42036 | |
90 | 177 | 84790, 84257 | FlhC; flagellar transcriptional activator | S. enterica serovar Typhimurium LT2 | 27 | 41/150 | AAB96640 | |
91 | 99 | 85086, 84787 | FlhD protein | Serratia liquefaciens | 28 | 28/97 | S61276 | |
92 | 182 | 85631, 85083 | Hypothetical protein | S. enterica serovar Typhi CT18 | 32 | 39/121 | NP 458645 | |
93 | 202 | 86226, 85618 | Hypothetical transmembrane protein | Ralstonia solanacearum | 39 | 26/66 | CAD17270 | |
94 | 289 | 87136, 86267 | Unknown protein | Cryptic prophage CP-933M; Escherichia coli O157:H7 | 35 | 27/76 | NP 286859 | |
96 | 215 | 87561, 88208 | Putative prophage repressor protein; similar to cI repressor | Yersinia pestis | 38 | 87/227 | NP 404832 |
ORFs not showing significant homology to corresponding proteins in the public databases are not shown. Nucleotide position from start to stop codon in the R391 sequence. Identity in percentage and amino acids (number identical relative to total number examined) as determined with BLAST and FASTA.
Nucleotide position from start to stop codon in the R391 sequence.
Identity in percentage and amino acids (number identical relative to total number examined) as determined with BLAST and FASTA.