TABLE 1.
Growth rates of B. subtilis ccpA mutant strains expressing the ilv-leu operon from its own or a xylose-inducible promotera
Strain (genotype) | Amino acid addition(s) | Generation time (min)b |
---|---|---|
QB5407 (ccpA) | None | 155 ± 7 |
CAA | 55 ± 1 | |
Xylose | 156 ± 12 | |
Met + xylose | 109 ± 1 | |
GP325 (ccpA PxylA::ilv-leu) | Met | NGc |
Met + CAA | 55 ± 5 | |
Met + xylose | 47 ± 1 | |
Xylose | 68 ± 0 |
Cells were grown at 37°C under vigorous agitation in CE-Glc minimal medium supplemented with caseine hydrolysate (CAA) (0.1% wt/vol), methionine (met) (0.002% wt/vol), or xylose (1.5% wt/vol) as indicated. The growth was monitored by measuring the optical density at 600 nm.
The generation times were determined from the growth of at least three independent cultures under each condition.
NG, no growth.