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. 2002 Sep;184(18):5151–5157. doi: 10.1128/JB.184.18.5151-5157.2002

FIG. 3.

FIG. 3.

SDS-PAGE analysis of rOrf4 expressed in and purified from E. coli. The rOrf4 protein is indicated by an arrow. Lane 1, molecular size markers; lane 2, crude extract from IPTG-induced E. coli BL21/pLysS harboring pET-orf4; lane 3, purified rOrf4 after DEAE-Sephacel anion-exchange chromatography; lane 4, crude extract from IPTG-induced E. coli BL21/pLysS harboring pET-3d. A sample containing 7 μg was separated on a 10 to 20% linear gradient gel, and the gel was stained with Coomassie brilliant blue G-250.