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VB synthesizing activity of rBarS1 for a series of A-factor-type substrates
Total carbon number in the C-2 side chain is indicated by n, with an asterisk indicating the substrate having terminal isopropyl moiety.
Each reaction was performed with purified rBarS1 (1.54 μg) at a substrate concentration of 1 mM and with 10 mM NADPH at 25°C in buffer A (without EDTA-Na2 [pH 7.5]) containing 0.5 M NaCl and 100 μM NADPH.
