FIG. 2.

RS comparisons. (A) Comparison of RS1 (top line) and RS2 (bottom line) sequences. Conserved nucleotides are boxed, and inverted repeat sequences are indicated with arrows. The putative transfer origin nick site is noted with the triangle. The putative Shine-Dalgarno (SD) sequences and the ATG start codons of the corresponding mob genes are also shown. The hatched bar indicates the sequence where amplification-related recombination occurs in vivo. (B) Sequence of the junction site of in vivo amplification products, using the 1.5-kb EcoRI fragment (see Fig. 3) as template to generate a PCR product from the indicated primers. The probable crossover point is indicated by the switch to bold letters.