TABLE 2.
Mobilization frequencies of pAMα1 and its deletion derivatives by pAM307 or its TraX or TraW mutants
| Plasmids in donors | Transfer frequency (UV202 to OG1SS)a
|
|
|---|---|---|
| pAD1 derivatives (Er/donor) | pAMα1 derivative (Tc/donor) | |
| pAM307, pAMα1 | 1 | 3.5 × 10−5 |
| pAM307, pAM8501 | 1 | 6.0 × 10−6 |
| pAM307, pAM8502 | 1 | 8.5 × 10−6 |
| pAM307, pAM8503 | 1 | 2.1 × 10−6 |
| pAM8130, pAMα1 | <10−8 | 1.5 × 10−5 |
| pAM8130, pAM8503 | <10−8 | <10−8 |
| pAM8131, pAMα1 | <10−8 | <10−8 |
| pAM8131, pAM8503 | <10−8 | <10−8 |
a
Transfer frequencies are expressed as the number of transconjugants per donor cell and represent the average of, at least, two independent experiments. Matings were conducted by using the UV202 host as the donor and OG1SS as the recipient. Er, erythromycin resistance; Tc, tetracycline resistance.