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Frequency of simple insertion or cointegration mediated by mini-ISY100
| Plasmida | tnpb | Frequency ofc:
|
|
|---|---|---|---|
| Simple insertion | Cointegration | ||
| pAU5 | + | 3.3 × 10−4 | <5.3 × 10−7 |
| pAU3 | − | <3.2 × 10−7 | <3.2 × 10−7 |
Donor plasmid carrying mini-ISY100.
Presence (+) or absence (−) of the IPTG-inducible transposase gene (tnp) in the pAU plasmid.
Frequencies of simple insertion and cointegration were calculated as described in Materials and Methods.
