TABLE 3.
Expression of Tn5-lacZ fusions in the bsgA bcsA double mutant during development
Fusion | Expression time (h)a | B- or C-signaling requirementb | Restored expression in bsgA bcsA double mutantc |
---|---|---|---|
Ω4521 | 2 | B | − |
Ω4455 | 2 | B | − |
Ω4494 | 2 | B | − |
Ω4273 | 5 | B | − |
Ω4411 | 6 | B | + |
Ω4427 | 7 | B, C | + |
Ω4414 | 12 | B, C | + |
Ω4435 | 25 | B, C | − |
Ω4401 | 25 | B, C | − |
Expression time is as defined by Kroos et al. (31) as the time after plating on starvation medium that developmental expression of the respective fusion occurs.
Indicates requirement for B or C signal for full expression of the respective fusion (29).
The peak level of β-galactosidase expression in the bsgA bcsA double mutant was comparable to that of the bsgA mutant alone. “+” indicates at least a fourfold higher β-galactosidase specific activity in the double mutant; “−” indicates less than a fourfold difference in specific activity levels.