TABLE 1.
Strains and plasmids
| Strain or plasmid | Genotype | Source or reference |
|---|---|---|
| Strains | ||
| TKW3205 | ΔkdpFABC Δatp706 thi rha lacZ nagA trkA405 trkD1 | 16 |
| DH5α | recA1 endA1 gyrA96 thi-1 relA1 hsdR17 (rK−, mK+) λ−supE44 F− φ80d lacZ ΔM15 Δ(lacZYA-argF)U169 deoR pho | GibcoBRL (Eggenstein, Germany) |
| Plasmids | ||
| pUC19 | 23 | |
| pSR3 | 3 | |
| pSR4 | kdpFABC | 17 |
| pSR5 | kdpFABC-kdpA:Q116R, derivative of pSR4 | 9 |
| pSD126 | kdpFABC-kdpA:G232D, derivative of pSR4 | 18 |
| pSM1 | kdpFABC, derivative of pSR4 | 10 |
| pSM2 | pSM1, derivative with KpnI site at position 806a | This study |
| pSM-kdpA:G232A | kdpA:G232A, derivative of pSM2 | This study |
| pSM-kdpA:G232S | kdpA:G232S, derivative of pSM2 | This study |
| pSM-kdpA:G233A | kdpA:G233A, derivative of pSM2 | This study |
| pSM-kdpA:G233S | kdpA:G233S, derivative of pSM2 | This study |
| pSM-kdpA:G233D | kdpA:G233D, derivative of pSM2 | This study |
| pSM-kdpA:G233Y | kdpA:G233Y, derivative of pSM2 | This study |
| pSM-kdpA:G234A | kdpA:G234A, derivative of pSM2 | This study |
| pSM-kdpA:G234S | kdpA:G234S, derivative of pSM2 | This study |
| pSM-kdpA:G234D | kdpA:G234D, derivative of pSM2 | This study |
a
Positions given are counted from the transcription start according to the study of Sugiura et al. (22).