TABLE 2.

Cold-specific stress proteins identified from 2-D gels of protein extracts of sporulating cells of B. subtilis PS 346^a

Cold shock protein	Molecular mass (kDa)	Isoelectric point	Identityb	Expression of cold shock proteins (% Spot volume compared to total gel spot volume)c
				Cold shock	Control
1	7.2 ± 0.2	4.82 ± 0.03	CspC	4.56 ± 0.2	1.28 ± 0.1
2	7.4 ± 0.3	4.23 ± 0.04	CspBd	6.81 ± 0.5	2.11 ± 0.1
3	7.3 ± 0.4	4.35 ± 0.04	CspBd	3.15 ± 0.2	0.65 ± 0.04
4	7.4 ± 0.3	4.51 ± 0.05	CspD	1.82 ± 0.1	0.44 ± 0.02
5	9.3 ± 0.5	4.32 ± 0.03		2.41 ± 0.1	0.38 ± 0.05
6	12.2 ± 0.6	5.13 ± 0.06	S6	1.58 ± 0.1	0.21 ± 0.02
7	14.9 ± 0.4	5.81 ± 0.07	PPiB	3.01 ± 0.2	0.38 ± 0.03
8	19.4 ± 0.5	4.61 ± 0.04		1.15 ± 0.2	0 ± 0
9	21.5 ± 0.5	5.35 ± 0.07		2.97 ± 0.2	0 ± 0
10	31.8 ± 0.6	5.54 ± 0.08	FbaA	2.36 ± 0.1	1.12 ± 0.2
11	33.6 ± 0.7	5.52 ± 0.05	EF-Ts	1.95 ± 0.1	0.87 ± 0.1
12	35.7 ± 0.5	4.53 ± 0.03		0.86 ± 0.05	0.32 ± 0.05
13	38.1 ± 0.3	5.64 ± 0.05	IIvC	3.65 ± 0.2	1.12 ± 0.1

^aIsoelectric point and molecular mass are estimated from migration in the 2-D gel.

^bThe identity of the proteins was determined by comparison of the N-terminal sequence to protein databases (SWISS-PROT and SubtiList).

^cData are the means ± standard deviations of three independent experiments.

^dCspB was identified as two spots on 2-D gels, the formylated and the deformylated form.