TABLE 3.
GS expression in adapted cultures
| Strain | Relevant genotype | GS transferase activity ina:
|
|
|---|---|---|---|
| Ggtrypb | GNgtryp | ||
| YMC10 | Wild type | 1,478 (4.1)c | 221 (8.7) |
| RB9060 | ΔglnB | 1,889 (4.2) | 1,516 (11.4) |
| Kc | ΔglnK | 1,661 (4.2) | 247 (9.8) |
| BKcd | ΔglnB ΔglnK | 1,864 (11.7) | 2,270 (11.9) |
| BKcBpB | ΔglnB ΔglnK Φ(glnBp-glnB) | 1,462 (3.9) | 180 (9.0) |
| BKcKpK | ΔglnB ΔglnK Φ(glnKp-glnK) | 1,910 (4.5) | 1,421 (11.4) |
| BKcBpK2d | ΔglnB ΔglnK Φ(glnBp-glnK) | 1,775 (12) | 2,005 (11.6) |
| BKcKpB2 | ΔglnB ΔglnK Φ(glnKp-glnB) | 1,676 (4.2) | 1,559 (11.4) |
| BKcApB2 | ΔglnB ΔglnK Φ(glnAp-glnB) | 1,010 (4.0) | 191 (7.4) |
| BKcApK2 | ΔglnB ΔglnK Φ(glnAp-glnK) | 1,594 (3.8) | 379 (9.7) |
a
Cultures were grown overnight, diluted to an optical density at 600 nm of ∼0.02, and grown at 30°C to an optical density at 600 nm of ∼0.5.
b
The media used were glucose-glutamine-tryptophan medium (Ggtryp) and glucose-ammonia-glutamine-tryptophan medium (GNgtryp). In all cases, tryptophan was present at a concentration of 0.004% (wt/vol), glucose was present at a concentration of 0.4% (wt/vol), and the nitrogen source was present at a concentration of 0.2% (wt/vol).
c
The number in parentheses is the adenylylation state expressed as the average number of adenylylated subunits per GS dodecamer.
d
Strain grew very poorly, with a doubling time of 4 h.