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. 2002 Oct;184(19):5508–5512. doi: 10.1128/JB.184.19.5508-5512.2002

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Copyright © 2002, American Society for Microbiology

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FIG. 2. — FepA globular domain deletions fractionate with the outer membrane. Specific gravity and NADH oxidase (NADHox) activity are provided for fractions from the strain expressing wild-type FepA; the mutants had similar profiles (not shown). Immunoblots of sodium dodecyl sulfate-11% polyacrylamide gels developed with α-FepA polyclonal antibody (13) at 1:5,000 are shown. (A) KP1394/pKP515 (FepA wild type); (B) KP1394/pKP516 (FepAΔ1-152); (C) KP1394/pKP517 (FepAΔ7-152); (D) KP1394/pKP518 (FepAΔ20-152); and (E) KP1394/pKP519 (FepAΔ17-150).