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FepA globular domain mutants do not cross-link to TonB. Immunoblot of cross-linked samples on a sodium dodecyl sulfate-11% polyacrylamide gel developed with anti-TonB monoclonal antibody 4F1 (19) at 1:5,000 is shown. W3110, fepA+ (chromosome); FepA−, KP1394; FepA+ (plasmid), KP1394/pKP515; fepAΔ1-152, KP1394/pKP516; fepAΔ7-152, KP1394/pKP517; fepAΔ20-152, KP1394/pKP518; and fepAΔ17-150, KP1394/pKP519.
