TABLE 2.
FepA globular domain deletion mutants are resistant to colicin B (ColB) and marginally sensitive to colicin D (ColD)
Strain | Genotype | Sensitivitya to
|
|
---|---|---|---|
ColB | ColD | ||
KP1411/pBAD24 | aroB fepA− | Rd | UDb |
KP1411/pKP515 | aroB fepA+ | 3.9 × 10−5 | 7.8 × 10−4 |
KP1411/pKP516 | aroB fepAΔ1-152 | R | 0.2c |
KP1411/pKP517 | aroB fepAΔ7-152 | R | 0.2c |
KP1411/pKP518 | aroB fepAΔ20-152 | R | 0.2c |
KP1411/pKP519 | aroB fepAΔ17-150 | R | 0.2c |
Results are expressed as the highest dilution at which clearing of the bacterial lawn was observed.
Undiluted colicin.
Almost undetectable zones of killing.
R, resistant.