FIG. 5.

Succinate inhibits the accumulation of raffinose. (A) Strain Rm1021 (wild type [w.t.]) was grown in M9 minimal medium plus 0.4% raffinose. A portion of the culture was exposed to 0.4% succinate for 1 h, and then the uptake of [³H]raffinose was measured in both the raffinose and the raffinose-plus-succinate cultures. Error bars indicate one standard error. (B) Strains Rm1021 and RB33 (lacR::Tn5-233) were grown in M9 minimal medium containing 0.4% raffinose plus 0.4% succinate. Succinate was present continuously as cells were grown in preparation for uptake measurements.