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. 2002 Oct;184(19):5410–5417. doi: 10.1128/JB.184.19.5410-5417.2002

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Copyright © 2002, American Society for Microbiology

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FIG. 2. — Phosphorylation pattern of HPr from wild-type B. thuringiensis subsp. israelensis and ptsH S45A mutant strain SR17. (A) Autoradiogram showing phosphorylation of HPr by HPr kinase and [γ-³²P]ATP at serine-45. Lane 1, HPr from B. thuringiensis subsp. israelensis phosphorylated by HPr kinase from B. thuringiensis subsp. israelensis; lane 2, HPr from SR17 phosphorylated by HPr kinase from SR17; lanes 3 and 4, B. thuringiensis subsp. israelensis recombinant HPr phosphorylated by HPr kinase from B. thuringiensis subsp. israelensis and SR17, respectively. (B) Western blot of nondenaturing polyacrylamide gel, showing phosphorylation of HPr by PEP and recombinant EI from B. subtilis at histidine-14. Lane 1, HPr from B. thuringiensis subsp. israelensis; lane 2, HPr from B. thuringiensis subsp. israelensis phosphorylated by EI and PEP; lane 3, HPr from SR17; lane 4, HPr from SR17 phosphorylated by EI and PEP.