FIG. 6.

mRNA profiles of σ³⁵, σ²⁸, and cry4A in B. thuringiensis subsp. israelensis (Bti) and SR17 (ptsH S45A) mutant during growth. Both strains were grown in G-medium, and cell samples were removed at different times. Total RNA was prepared from these cells and probed with cry4A-, σ³⁵-, or σ²⁸-specific probes to determine the transcript level. (A) Profile of cry4A-specific mRNA. (B) Profile of σ³⁵-specific mRNA. (C) Profile of σ²⁸-specific mRNA. Slots 1 to 8, samples removed at 6 h, 8 h, 10 h, 12 h, 14 h, 16 h, 18 h, and 20 h, respectively.