TABLE 1.
Strains and plasmids used in this study
| Plasmid or strain | Relevant characteristics | Reference |
|---|---|---|
| Plasmids | ||
| pSR2 | pGEM-T easy containing a 293-bp PCR-amplified ptsH | 18 |
| pSR15 | pGEM-T easy containing 293-bp ptsH S45A | This work |
| pSR16 | pGEM-T easy containing 293-bp ptsH S45A and kanamycin resistance cassette | This work |
| pSR18 | pGEM-T easy containing 700-bp B. thuringiensis subsp. israelensis σ33 | This work |
| pSR19 | pGEM-T easy containing 700-bp B. thuringiensis subsp. israelensis σ28 | This work |
| pDG783 | pSB118 containing kanamycin cassette at HindIII site | 14 |
| Strains | ||
| SR2 | E. coli DH5α cells harboring 293-bp ptsH gene of B. thuringiensis subsp. israelensis in pGEM-T easy | 18 |
| SR15 | E. coli DH5α cells containing 293-bp ptsH S45A gene in pGEM-T easy | This work |
| SR16 | E. coli RR1 cells containing 293-bp ptsH S45A and kanamycin cassette in pGEM-T easy | This work |
| SR17 | B. thuringiensis subsp. israelensis with ptsH S45A integrated downstream of wild-type ptsH promoter in chromosome | This work |
| E. coli RR1 | hsdS20 (r−B m+B) supE44 ara-14 proA2 rspL20(Strr) lacY1 galK2 xyl-5 mtl-1 supE44 | Promega |
| E. coli XL1-Blue | recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F′ proAB lacIqZΔM1515 Tn10(Tetr)] | Stratagene |