Plasmids |
|
|
pSR2 |
pGEM-T easy containing a 293-bp PCR-amplified ptsH
|
18 |
pSR15 |
pGEM-T easy containing 293-bp ptsH S45A |
This work |
pSR16 |
pGEM-T easy containing 293-bp ptsH S45A and kanamycin resistance cassette |
This work |
pSR18 |
pGEM-T easy containing 700-bp B. thuringiensis subsp. israelensis σ33
|
This work |
pSR19 |
pGEM-T easy containing 700-bp B. thuringiensis subsp. israelensis σ28
|
This work |
pDG783 |
pSB118 containing kanamycin cassette at HindIII site |
14 |
Strains |
|
|
SR2 |
E. coli DH5α cells harboring 293-bp ptsH gene of B. thuringiensis subsp. israelensis in pGEM-T easy |
18 |
SR15 |
E. coli DH5α cells containing 293-bp ptsH S45A gene in pGEM-T easy |
This work |
SR16 |
E. coli RR1 cells containing 293-bp ptsH S45A and kanamycin cassette in pGEM-T easy |
This work |
SR17 |
B. thuringiensis subsp. israelensis with ptsH S45A integrated downstream of wild-type ptsH promoter in chromosome |
This work |
E. coli RR1 |
hsdS20 (r−B m+B) supE44 ara-14 proA2 rspL20(Strr) lacY1 galK2 xyl-5 mtl-1 supE44
|
Promega |
E. coli XL1-Blue |
recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F′ proAB lacIqZΔM1515 Tn10(Tetr)] |
Stratagene |